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Related post: October 1. 1982 through September 30. 1983 TITLE OF PROJECT (80 characten or leu. Title mutt fit on one line between the border*.) MOLECULAR VIROLOGY OF HEPATITIS A VIRUS (HAV) PRINCIPAL INVESTIGATOR (List other prof Grifulvin V Micr ettional pertonnel on subaequent paget.) (Name, title, laboratory, and inttltute affiliation) John Ticehurst. M.D., Medical Officer, Hepatitis Viruses Section, LID NIAID COOPERATING UNITS (if any) Columbia University (Dr. V. Racaniello), Massachusetts Institute of Technology (Dr. D. Baltimore) LAB/BRANCH LABORATORY OF INFECTIOUS DISEASES SECTION HEPATITIS VIRUSES SECTION INSTITUTE AND LOCATION NIAID, NIH, BETHESDA, MARYLAND 20205 TOTAL MANYEARS; 1.6 PROFESSIONAL: 0.6 1.0 CHECK APPROPRIATE BOX(ES) CH (a) Human subjects □ (a1) Minors □ (a2) Interviews □ (b) Human tissues C25 (c) Neither SUMMARY OF WORK (Use standard unreduced type. Do not exceed the ipace provided.) Double-stranded cDNA fragments were synthesized from hepatitis A virus (HAV) RNA and inserted into the Pst I site of Buy Grifulvin Online pBR322. The identity of cloned cDNA was established by demonstrating its hydridization to RNA from HAV-infected tissue culture cells but not to RNA from uninfected cells. Genomic length RNA of approximately 7500 nucleotides was the predominant species that hybridized with the HAV cloned DNA. Restriction endonuclease digestion and Buy Grifulvin hybridization between subgenomic fragments yielded a map of overlapping cloned cDNAs which includes 99% of the viral genome. A partial sequence from the 3' end of the genome contained 890 bases in an open reading frame preceding stop codons, 60 bases of a noncoding region, and a tract of poly(A). 22-28 PHS 6040 (Rev. 1/83) GPO 895-100 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL Grifulvin V 500 RESEARCH PROJECT PROJECT NUMBER ZOl AI 00310-02 LID PERIOD COVERED Ort.nhPr 1, 1982 through .Sppt.PmhPr 30, iqft.? TITLE OF PROJECT (80 characterB or leee. Title must fit on one line between the borden.) HYBRIDOMA ANTIRODTE.S TO PATHOGENIC VIRHSF.S PRINCIPAL INVESTIGATOR (List other professional personnel on aubaequent pages.) (Name, title, laboratory, and institute affiliation) Stephen M. Feinstone. M.D.. Medical Officer. HVS LID NIAID COOPERATING UNITS (if any) NIAID, NIH, Bethesda, Maryland (Dr. A. Fauci) LAB/BRANCH LABORATORY OF INFECTIOUS DISEASES SECTION HEPATITIS VIRUSES SECTION INSTITUTE AND LOCATION NIAID, NIH, BETHESDA, MARYLAND 20205 TOTAL MANYEARS: 1.6 PROFESSIONAL; 1.6 JUL CHECK APPROPRIATE BOX(ES) □ (a) Human subjects □ (b) Human tissues □ (a1) Minors □ (a2) Interviews [3 (c) Neither SUMMARY OF WORK (Vse standard unreduced type. Do not exceed the tpace provided.) Attempts to produce monoclonal antibody to hepatitis A virus are in progress. The development of new serologic tests for detection of monoclonal anti-HAV will simplify the procedure. Two specific monoclonal antibodies have been produced and are Grifulvin V being analyzed. 22-29 PHS 6040 (Rev. 1/83) GPO 895-100 0EPART1MENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00311-02 LID PERIOD COVERED nrtnhpy 1, IQft? thr n.irjh <;pptpmhpr .'^0, 1983 TITLE OF PROJECT (80 character! or leBS. Title muit fit on one tine between the bordert.) SFARfH FOR NFW HFPATTTTS AGENTS PRINCIPAL INVESTIGATOR (Lift other profettional personnel on aubtequent paget.) (Name, title, laboratory, and inititute affiliation) Robert H. Purcell. M.D. Head. Hepatitis Viruses Section LID NIAID COOPERATING UNITS (if any) Clinical Center Blood Bank (Dr. H. Alter); National Institute of Virology, Pune, India (Dr. K. Pavri); Medical Buy Grifulvin V College of Srinagar, India (Dr. M. S. Khuroo); Mt. Sinai Hospital (Dr. H. Popper).
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